Journal: International Journal of Molecular Sciences

Article Title: Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform, CellSaic, in a Rat Congenital Cleft-Jaw Model

doi: 10.3390/ijms22179478

Figure Lengend Snippet: Surface markers of rBMSCs and rDPSCs. Flow cytometry analyses revealed that both cells were positive for CD90, CD73, and CD44 but negative for CD45.

Article Snippet: Using FACSVerse (BD Biosciences, Franklin Lakes, NJ, USA) to analyze the cell suspension, we evaluated the following surface antigens with antibodies: CD44 (Cat#12–0444–80, Thermo Fisher Scientific Inc., Waltham, MA, USA), CD45 (Cat#554878, BD Pharmingen, San Diego, CA, USA), CD73 (Cat#bs-4834r-pe, Bioss Antibodies Inc., Boston, MA, USA), and CD90 (Cat#202526, BioLegend, San Diego, CA, USA).

Techniques: Flow Cytometry

Journal: Medicine

Article Title: Asperosaponin VI induces osteogenic differentiation of human umbilical cord mesenchymal stem cells via the estrogen signaling pathway

doi: 10.1097/MD.0000000000032344

Figure Lengend Snippet: Flow cytometry detection of UC-MSC surface markers. (A) Anti-CD44 monoclonal antibody. (B) Anti-CD31 monoclonal antibody. (C) Anti-CD45 monoclonal antibody. (D) Anti-CD73 monoclonal antibody. UC-MSC = umbilical cord mesenchymal stem cells.

Article Snippet: Anti-CD44-PE, anti-CD31-PE, anti-CD73-PE, and anti-CD45-PE were purchased from SinoBiological (Beijing, China), and the reverse transcription kit was purchased from Axygen (San Francisco, CA).

Techniques: Flow Cytometry

Journal: Journal of inflammation (London, England)

Article Title: Mesenchymal stem cell-derived exosomal CBLB ameliorates infantile pneumonia progression probably by ubiquitinating MAPK14.

doi: 10.1186/s12950-025-00450-0

Figure Lengend Snippet: Fig. 1 The identification of MSCs and the exosomes isolated from MSCs. A and B Flow cytometry analysis was performed to analyze the expression of hematopoietic markers of MSCs (CD11b, CD45 and CD34) and their marker proteins (CD105, CD90, and CD73). C The morphology of MSCs and their abili ties to differentiate into adipocytes and osteoblasts were observed or analyzed using a microscope, oil red O staining experiment, and ALP assay. D-F The identification of the isolated exosomes from MSCs by TEM, NTA analysis and western blotting assay

Article Snippet: The cells were incubated with PE-conjugated fluorescent antibodies against CD11b (E-AB-F1081D, Elabscience, Wuhan, China), CD45 (E-AB-F1137D, Elabscience), CD34 (E-ABF1143D, Elabscience), CD105 (E-AB-F1310D, Elabscience), CD90 (E-AB-F1167D, Elabscience), and CD73 (E-AB-F1242D, Elabscience).

Techniques: Isolation, Flow Cytometry, Expressing, Marker, Microscopy, Staining, ALP Assay, Western Blot

Journal: iScience

Article Title: Identification of optimal conditions for human placental explant culture and extracellular vesicle release

doi: 10.1016/j.isci.2023.108046

Figure Lengend Snippet:

Article Snippet: Human anti-CD73 PE , Cellarcus Biosciences , Cat# CBS81-PE-100T.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Proliferation Assay, LDH Cytotoxicity Assay, Software