Journal: Stem Cell Research & Therapy

Article Title: Lung delivery of MSCs expressing anti-cancer protein TRAIL visualised with 89 Zr-oxine PET-CT

doi: 10.1186/s13287-020-01770-z

Figure Lengend Snippet: MSCTRAIL show time- and amount-dependent sensitivity to 89 Zr-oxine labelling but retain MSC phenotype. MSCTRAIL cells were labelled from frozen with 89 Zr-oxine between 79 and 332 kBq/10 6 . Cells show reduced proliferation with increasing amount as indicated by metabolism of ATP ( a ) and NADH ( b ). Error bars show standard deviation (SD). * p < 0.05, ** p < 0.01, *** p < 0.001 2-way ANOVA with Dunnett’s multiple comparisons test vs PBS control. c Retention of 89 Zr oxine decreases over time ( n = 4). The 2-way ANOVA analysis showed that the majority of variation (67.5%) was due to time ( p < 0.0001), with amount having a significant ( p = 0.015) but small effect (3% of variation) on activity retention. d MSCTRAIL retain their MSC phenotype (CD45-ve, CD73, CD90, and CD105+ve), post-radiolabelling with 89 Zr-oxine (amount shown 332 kBq/10 6 cells)

Article Snippet: For immunophenotyping of MSCs, the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770, 130-104-224, Miltenyi Biotec), CD105 (APC, 130-094-926, Miltenyi Biotec), CD90 (PE, 561970, BD Biosciences), CD34 (FITC, 560942, BD Biosciences), CD45 (FITC, 560976, BD Biosciences), and HLA-DR (FITC, 555560, BD Biosciences).

Techniques: Standard Deviation, Control, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Comparison of Osteogenic Potentials of Dental Pulp and Bone Marrow Mesenchymal Stem Cells Using the New Cell Transplantation Platform, CellSaic, in a Rat Congenital Cleft-Jaw Model

doi: 10.3390/ijms22179478

Figure Lengend Snippet: Surface markers of rBMSCs and rDPSCs. Flow cytometry analyses revealed that both cells were positive for CD90, CD73, and CD44 but negative for CD45.

Article Snippet: Using FACSVerse (BD Biosciences, Franklin Lakes, NJ, USA) to analyze the cell suspension, we evaluated the following surface antigens with antibodies: CD44 (Cat#12–0444–80, Thermo Fisher Scientific Inc., Waltham, MA, USA), CD45 (Cat#554878, BD Pharmingen, San Diego, CA, USA), CD73 (Cat#bs-4834r-pe, Bioss Antibodies Inc., Boston, MA, USA), and CD90 (Cat#202526, BioLegend, San Diego, CA, USA).

Techniques: Flow Cytometry

Journal: Medicine

Article Title: Asperosaponin VI induces osteogenic differentiation of human umbilical cord mesenchymal stem cells via the estrogen signaling pathway

doi: 10.1097/MD.0000000000032344

Figure Lengend Snippet: Flow cytometry detection of UC-MSC surface markers. (A) Anti-CD44 monoclonal antibody. (B) Anti-CD31 monoclonal antibody. (C) Anti-CD45 monoclonal antibody. (D) Anti-CD73 monoclonal antibody. UC-MSC = umbilical cord mesenchymal stem cells.

Article Snippet: Anti-CD44-PE, anti-CD31-PE, anti-CD73-PE, and anti-CD45-PE were purchased from SinoBiological (Beijing, China), and the reverse transcription kit was purchased from Axygen (San Francisco, CA).

Techniques: Flow Cytometry